Figure 3

Immunohistochemistry of combined ATRX and ATRY in the adult tammar wallaby testis, and for ATRX in the adult tammar ovary. Antibody staining is shown by brown/red staining and tissues are counterstained blue with haematoxylin. In the adult testis, ATRX/Y was predominantly localized at the periphery of the seminiferous tubules (A). ATRX was nuclear in the primary spermatogonia but was absent from the Sertoli cells (C). Protein was also detected in the interstitial cells that resemble Leydig cells (E). In the adult ovary ATRX was seen in the granulosa cells of developing follicles (B) at all stages of growth, but not in primordial follicles (D, F). ATRX was also detected in the steroidogenic theca cells (D) of antral follicles, in the corpus luteum (H), in the ovarian surface epithelium (F) and in the oocytes themselves (F). There was no staining in the antibody negative control (G). SG - spermatogonia, GC - germ cell, SC - Sertoli cell, LC - Leydig cell, ST - seminiferous tubule, AF - antral follicle, T - theca, 2°F - secondary follicle, PrF - primordial follicle, GC - granulosa cell, O - oocyte, SE - ovarian surface epithelium, Scale bars; A,D,G,H = 200 μm, B = 500 μm, C, E, F = 50 μm.