Figure 1

Whole mount in situ hybridization of ATRY in the developing tammar testis (B, D) and ATRX in the developing mouse testis (A, C) and mouse and tammar ovary (E, F). Stages shown for mouse and tammar are at equivalent developmental time points. mRNA distribution is shown by dark blue/purple staining and tissues are bleached white. ATRX and ATRY mRNA transcripts were diffuse and dispersed throughout the indifferent gonad and to a lesser extent, the mesonephros (A,B). Once testicular differentiation began, ATRX in mouse and ATRY in tammar were confined to the developing testis cords and was absent in the regressing mesonephros (C, D). In the ovary, ATRX mRNA showed a punctate localization in the cortexin both mouse (E) and tammar ( F), indicative of germ cell staining. M - mesonephros, G - gonad, O - ovary, T - testis, scale bar = 500 μm.