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Figure 6 | BMC Developmental Biology

Figure 6

From: Structure and epitope distribution of heparan sulfate is disrupted in experimental lung hypoplasia: a glycobiological epigenetic cause for malformation?

Figure 6

Spatiotemporal distribution of HS epitopes is abnormal in hypoplastic lungs. In normal development, a gradient of EV3C3V epitope distribution is observed in the mesenchyme of E15.5 - E17.5 lungs, with high epitope levels in sub-epithelial mesenchyme around distal airways (arrowhead) and lower levels around proximal airways. This organised gradient of EV3C3V epitope distribution is lost in lungs of nitrofen treated rats, which display the structure at a high level throughout the mesenchyme at E15.5 - E17.5. At E19.5 - E21.5, EV3C3V epitope levels and distribution are comparable to control lungs. Hypoplastic lungs from rats with nitrofen-induced left sided CDH and control lungs from rats fed olive oil alone were probed with EV3C3V followed by rabbit VSV-G tag antibody and FITC conjugated goat anti-rabbit IgG. Scale bar represents 10 μm and all images are the same magnification. (aw) airway, (me) mesenchyme.

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