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Figure 4 | BMC Developmental Biology

Figure 4

From: Structure and epitope distribution of heparan sulfate is disrupted in experimental lung hypoplasia: a glycobiological epigenetic cause for malformation?

Figure 4

HS epitope distribution in pulmonary vasculature. HS3B7V shows a unique pattern of vascular staining in fetal lungs, specifically highlighting the outer tunica media of arterial walls (arrow) and leaving the inner tunica media unlabelled. In addition, this antibody does not stain pulmonary veins. (Some weak, nuclear staining was observed with HS3B7V on occasion, including in veins. However, this was not sensitive to heparinase digestion and is therefore non-specific staining). The remaining antibodies highlight the entire tunica media layer in the walls of both arteries and veins (only AO4B08V, HS4E4V and EV3C3V are shown, however, HS3A8V and HS4E4V display comparable blood vessel staining). Fetal rat lungs were probed with HS antibodies followed by rabbit VSV-G tag antibody and FITC conjugated goat anti-rabbit IgG. Scale bar represents 10 μm and all images are the same magnification. (A) artery, (V) vein, (aw) airway, (m) media.

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BMC Developmental Biology

ISSN: 1471-213X

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