Figure 4
From: The Sphingosine-1-phospate receptor 1 mediates S1P action during cardiac development
S1P receptor agonists do not increase cell death in AV canal cultures. Cell viability was assessed following S1P1R receptor agonist treatment. (A, D) AV canal explant treated with vehicle control (methanol). (B, E) explants treated with 0.5 μM KRP203. (C, F) explants treated with 1.0 μM FTY720. (A, B, C) only live cells are labeled with calcein AM and appear green, where as (D, E, F) only dead cells are labeled with ethidium homodimer-1 and appear red. Dead cells are seen around the edges of the explants and in the muscle explants but the majorities of cells fluoresce green and are alive under all treatments. Scale bar = 200 μm.