Figure 2
From: The Sphingosine-1-phospate receptor 1 mediates S1P action during cardiac development
S1P 1 R protein is localized to myocardial and endocardial cells in the developing heart. Wild type C57Bl/6 embryo sections were immunostained with (A, C, E) control normal rabbit IgG or (B, D, F) a rabbit polyclonal antibody against S1P1R. A secondary antibody conjugated to hydrogen peroxidase was used in conjunction with a Vector SG substrate color reaction kit to produce a blue-grey precipitate wherever S1P1R was expressed. Sections were counterstained with nuclear fast red to label the nuclei in each cell. S1P1R expression was observed in the myocardium (M) of both the atrium (At) and ventricle (V) as well as in endothelial cells (arrows) in both (A, B) E10.5 and (C, D) E12.5 hearts. (E, F) E10.5 embryonic sections, at the level of the somites, depicting vascular endothelial cells (arrow heads) stained with anti-S1P1R antibodies. (A, B, C, D) Scale bar = 100 μm, (A and B inserts and E, F) scale bar = 50 μm.