Figure 2

Knockdown of xPAPC leads to otocyst deformation. (A, A', B, C, C', D) ISH for Tbx2 shows an altered expression pattern at the injected side (A', C') compared to the wildtype side (A, C). A severe phenotype is shown in (A, A', B), a mild phenotype in (C, C', D). Corresponding transversal sections are presented in (B, D). Note the infolding epithelium at the injected side, arrowhead. (E, E', F) Rescue by coinjection of 500 pg FL-PAPC RNA, (E) wildtype, (E') PAPC Mo coinjected with full-length PAPC, (F) corresponding transversal section. (G) Statistical evaluation of xPAPC depletion and rescue experiments at stage 26. The embryos are single side injected and phenotypes were examined via Tbx2 ISH. FL-PAPC RNA was coinjected in increasing amounts (100 pg, 250 pg, 500 pg), also a control Morpholino was injected to eliminate the possibility of unspecific effects. The data are normalized relating to uninjected wildtype embryos. FL, full-length; Mo, antisense morpholino; n, number of embryos. Injected side is indicated by asterisks in transversal sections. Scale bar (A') 500 μm, (B) 100 μm. ** = p < 0,01