Figure 2

Transcriptional activation of teneurin-1 promoter reporter constructs by EMX2. (A) NIH3T3 cells were transfected with a flag-myc-tagged EMX2 construct or an empty vector and tested for expression by Western blot of whole cell extract with anti-Flag. (B) Immunostaining of the cells with anti-Flag reveals the nuclear accumulation of the EMX2 protein in the nuclei of the cells. (C) HEK293 cells were transfected with EMX2 or empty vector control and co-transfected with different promotor constructs as indicated. The graph shows relative SEAP values for one representative experiment performed in triplicates (n = 5). The activity obtained with the Promotor 1 construct is arbitrarily set to 1. Error bars display standard deviation of the mean. The sequences of the region harboring the putative binding site and the mutation used (mut Promoter 2) are shown in the box above the bar graph.