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Figure 4 | BMC Developmental Biology

Figure 4

From: Expression of miR-1, miR-133a, miR-133b and miR-206 increases during development of human skeletal muscle

Figure 4

MyoD induces myotube formation in human foetal muscle cells. (A) Overexpression of endogenous MyoD levels were detected by Western blotting in 12-week old (i) and 14-week old (ii) muscle cells, transfected with the MyoD adenovirus (AdM), compared to adenovirus-control transfected cells (AdC) and untransfected cells (Un). (B) Immunocytochemistry of the myogenic marker myosin heavy chain (MyHC) showed a marked increase in myotube formation in cells overexpressing MyoD, compared to control cells. Nuclei were stained with DAPI. Scale bar, 0.8 mm. (C) Overexpression of MyoD resulted in increased levels of MyHC levels, as detected by Western blotting, in 12-week old (i) and 14-week old (ii) muscle cells, compared to control cells.

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BMC Developmental Biology

ISSN: 1471-213X

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