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Figure 1 | BMC Developmental Biology

Figure 1

From: Expression of miR-1, miR-133a, miR-133b and miR-206 increases during development of human skeletal muscle

Figure 1

Muscle cell differentiation increases during the late stages of foetal development. (A) Muscle cell differentiation was detected in all three human myoblast cell lines (12-week old, 14-week old and newborn) by immunocytochemistry against myosin heavy chain (MyHC). DAPI was used to stain cell nuclei. Scale bar, 0.8 mm. (B) Muscle formation was characterized by measuring or calculating (i) the average number of myotubes per area (initial cell fusion), (ii) the percentage of nuclei present in myotubes compared to the total number of nuclei present in each area (fusion index %) and (iii) the average number of nuclei per myotube (myotube maturation) in total 10 areas of cells.

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BMC Developmental Biology

ISSN: 1471-213X

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