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Table 2 Identification of abundant phosphoproteins in P. vexillosa during metamorphosis by MALDI-TOF MS.

From: Phosphoproteome analysis during larval development and metamorphosis in the spionid polychaete Pseudopolydora vexillosa

Spot No. Acc No.a) Protein name b) MW(kDa)c)Obs./Theo. pIc)Obs./Theo. PM/SC (%) Biological process
33 Contig0667618/gi|291243919 Nuclear ribonucleoprotein 36/34 6.5/6.5 16/22 rRNA processing
34 Contig56810076 Beta-actin 20/19 5.7/5.3 10/28 Cellular structure protein
35 gi|149912747 ABC transporter 33/33 5.8/5.4 7/29 Hormone response regulation
36 gi|116740271 Alpha-tubulin 43/43 6.9/5.9 10/18 Cytokinesis, cell division
37 GG70GXE04JMNIB_2/gi|212449 Myosin heavy chain 60/15 6.6/5.0 03/44 Myogenesis, muscle contraction
38 gi|131573157 Alpha-tubulin 35/45 6.5/5.7 7/25 Cytokinesis, cell division
39 gi|161072 Alpha-tubulin 40/50 5.7/4.9 9/10 Cytokinesis, cell division
40 GG70GXE04H5QQL_10/gi|116250948 Conserved hypothetical protein 40/16 5.5/5.6 4/25 Structural reorganization
41 Contig04504_14/gi|4481960 Polydom protein 40/15 5.5/4.7 12/21 Protein-protein interaction, adhesion
42 F5K2Q4C01CF4OT_4/gi|467215 Actin beta 50/41 5.4/5.5 11/33 Cellular structure protein
43 GG70GXE04JCRO8_11/gi|37528876 Actin 50/41 4.3/5.5 11/43 Cellular structure protein
44 Contig02678_14/gi|4468655 Intermediate Filamant A 70/71 5.6/5.5 16/20 Structural integrity, cell motility
45 gi|169824521 DNA primase 50/69 6.0/6.2 9/17 DNA replication
46 gi|161072 Alpha-tubulin 48/50 6.0/6.4 5/49 Cytokinesis, cell division
47 gi|38047815 Alpha Tub84B 18/28 5.4/5.3 14/25 Cytokinesis, cell division
48 gi|38047815 Alpha Tub84B 18/28 5.4/5.3 9/25 Cytokinesis, cell division
49 Contig10935_1/gi|168761509 Splicing factor 3A subunit 1 16/10 5.6/10 4/46 mRNA processing, splicing
50 gi|131573157 Alpha tubulin 15/10 5.7/5.7 8/57 Cytokinesis, cell division
51 gi|197129678 Tubulin beta 2 6/26 4.5/4.4 4/21 Cytokinesis, cell division
52 gi|999627 Epsilon trypsin 17/9 4.8/6.6 5/46 Proteolysis
53 Contig06726_6/gi|148225538 Tyrosine 3-monooxygenase 35/30 4.5/4.7 4/26 Muscle contraction
54 Contig13459_11/gi|4481960 Low-density lipoprotein receptor 33/22 4.5/4.6 11/31 Cholesterol homeostasis
55 gi|78190577 Beta-tubulin 55/43 5.3/5.7 11/37 Cytokinesis, cell division
56 gi|159400261 Alpha-tubulin 60/47 5.3/5.2 5/18 Cytokinesis, cell division
57 Contig56810076/gi|37528876 Actin, cytoplasmic 53/45 5.5/5.3 9/28 Cellular structure protein
58 gi|178045 Gamma-actin 29/26 5.5/5.7 12/25 Cellular structure protein
59 Contig56810077/gi|37528876 Actin, cytoplasmic A3 32/45 5.7/5.5 7/18 Cellular structure protein
60 gi|47117349 Tropomyosin 35/39 4.5/4.6 8/19 Muscle degeneration and differentiation
  1. a) Accession numbers are from the NCBInr and in-house P. vexillosa transcriptomic databases. b) For positive identification, the score had to be over the significance threshold level (p < 0.05). c) Observed (Obs.) MW and pI values were estimated from 2-DE gels and Theoretical (Theo.) MW and pI values were derived from a database search by MASCOT. PM: number of peptides matching the protein sequence; SC: sequence coverage.