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Table 2 Identification of abundant phosphoproteins in P. vexillosa during metamorphosis by MALDI-TOF MS.

From: Phosphoproteome analysis during larval development and metamorphosis in the spionid polychaete Pseudopolydora vexillosa

Spot No.

Acc No.a)

Protein name b)

MW(kDa)c)Obs./Theo.

pIc)Obs./Theo.

PM/SC (%)

Biological process

33

Contig0667618/gi|291243919

Nuclear ribonucleoprotein

36/34

6.5/6.5

16/22

rRNA processing

34

Contig56810076

Beta-actin

20/19

5.7/5.3

10/28

Cellular structure protein

35

gi|149912747

ABC transporter

33/33

5.8/5.4

7/29

Hormone response regulation

36

gi|116740271

Alpha-tubulin

43/43

6.9/5.9

10/18

Cytokinesis, cell division

37

GG70GXE04JMNIB_2/gi|212449

Myosin heavy chain

60/15

6.6/5.0

03/44

Myogenesis, muscle contraction

38

gi|131573157

Alpha-tubulin

35/45

6.5/5.7

7/25

Cytokinesis, cell division

39

gi|161072

Alpha-tubulin

40/50

5.7/4.9

9/10

Cytokinesis, cell division

40

GG70GXE04H5QQL_10/gi|116250948

Conserved hypothetical protein

40/16

5.5/5.6

4/25

Structural reorganization

41

Contig04504_14/gi|4481960

Polydom protein

40/15

5.5/4.7

12/21

Protein-protein interaction, adhesion

42

F5K2Q4C01CF4OT_4/gi|467215

Actin beta

50/41

5.4/5.5

11/33

Cellular structure protein

43

GG70GXE04JCRO8_11/gi|37528876

Actin

50/41

4.3/5.5

11/43

Cellular structure protein

44

Contig02678_14/gi|4468655

Intermediate Filamant A

70/71

5.6/5.5

16/20

Structural integrity, cell motility

45

gi|169824521

DNA primase

50/69

6.0/6.2

9/17

DNA replication

46

gi|161072

Alpha-tubulin

48/50

6.0/6.4

5/49

Cytokinesis, cell division

47

gi|38047815

Alpha Tub84B

18/28

5.4/5.3

14/25

Cytokinesis, cell division

48

gi|38047815

Alpha Tub84B

18/28

5.4/5.3

9/25

Cytokinesis, cell division

49

Contig10935_1/gi|168761509

Splicing factor 3A subunit 1

16/10

5.6/10

4/46

mRNA processing, splicing

50

gi|131573157

Alpha tubulin

15/10

5.7/5.7

8/57

Cytokinesis, cell division

51

gi|197129678

Tubulin beta 2

6/26

4.5/4.4

4/21

Cytokinesis, cell division

52

gi|999627

Epsilon trypsin

17/9

4.8/6.6

5/46

Proteolysis

53

Contig06726_6/gi|148225538

Tyrosine 3-monooxygenase

35/30

4.5/4.7

4/26

Muscle contraction

54

Contig13459_11/gi|4481960

Low-density lipoprotein receptor

33/22

4.5/4.6

11/31

Cholesterol homeostasis

55

gi|78190577

Beta-tubulin

55/43

5.3/5.7

11/37

Cytokinesis, cell division

56

gi|159400261

Alpha-tubulin

60/47

5.3/5.2

5/18

Cytokinesis, cell division

57

Contig56810076/gi|37528876

Actin, cytoplasmic

53/45

5.5/5.3

9/28

Cellular structure protein

58

gi|178045

Gamma-actin

29/26

5.5/5.7

12/25

Cellular structure protein

59

Contig56810077/gi|37528876

Actin, cytoplasmic A3

32/45

5.7/5.5

7/18

Cellular structure protein

60

gi|47117349

Tropomyosin

35/39

4.5/4.6

8/19

Muscle degeneration and differentiation

  1. a) Accession numbers are from the NCBInr and in-house P. vexillosa transcriptomic databases. b) For positive identification, the score had to be over the significance threshold level (p < 0.05). c) Observed (Obs.) MW and pI values were estimated from 2-DE gels and Theoretical (Theo.) MW and pI values were derived from a database search by MASCOT. PM: number of peptides matching the protein sequence; SC: sequence coverage.