Phosphoproteome analysis during larval development and metamorphosis in the spionid polychaete Pseudopolydora vexillosa

Table 1 Identification of Differentially Expressed Proteins in P. vexillosa during Metamorphosis by MALDI-TOF MS

Spot No.	Acc No.^a)	Protein name ^b)	MW(kDa)^c)Obs./Theo.	pI^c)Obs./Theo.	PM/SC (%)	Phosphorylation variation ratios			Biological process
						PRE	COM	META
1	Contig08739_26/gi\|224049395	Enolase-phosphatase E1	230/13	3.7/4.2	13/47	1.78	9.25	0.19	Amino-acid biosynthesis
3	GG70GXE04J1FKM_1/gi\|198433617	Glutaredoxin	29/25	4.5/9.6	10/19	1.12	20.53	0.27	Redox metabolism, oxidative stress
5	Contig06643_7/gi\|126116628	Paramyosin	34/30	5.5/5.1	10/38	1.17	0.33	0.09	Myofibril assembly, muscle contraction
6	gi\|91085405	GA20008-PA	32/25	5.8/10	6/25	1.07	1.37	0.18	RNA processing
11	GG7OGXE04JZMIN_9/gi\|194214808	Fatty acid binding protein 1, adipocyte	30/20	6.0/8.2	10/44	1.01	10.05	0.11	Energy storage, inflammation
15	GG70GXE04JCRO8_1/gi\|37528876	actin	14/10	5.7/4.9	6/44	0.74	6.38	0.08	Cellular structure
27	Contig00736_21/gi\|189007782	Myosin heavy chain	66/100	5.6/5.1	18/15	2.09	47.30	0.20	Myogenesis, muscle contraction
28	Contig00736_21/gi\|189007782	Myosin heavy chain	63/109	5.1/5.8	8/14	0.82	2.05	0.16	Myogenesis, muscle contraction
29	Contig02680_11/gi\|4468655	Intermediate Filament A	60/57	5.5/5.3	9/26	0.12	9.27	0.25	Structural integrity, cell motility
32	gi\|182623856	Beta actin	18/25	5.6/5.3	7/34	1.07	3.32	0.69	Cellular structure

a) Accession numbers are from the NCBInr and in-house P. vexillosa transcriptomic databases. b) For positive identification, the score had to be over the significance threshold (p < 0.05). c) Observed (Obs.) MW and pI values were estimated from 2-DE gels and Theoretical (Theo.) MW and pI values were derived from a database search by MASCOT. PM: number of peptides matching the protein sequence; SC: sequence coverage.

ISSN: 1471-213X