Figure 5

PRE-1 is sufficient to enhance reporter expression from photoreceptor-specific promoters. A. Schematic of the promoter-reporter regions of chimeric constructs. Three copies of PRE-1 (red boxes) are sufficient to enhance expression from both cone and rod promoter fragments, whereas three copies of PRE-1-rho (navy boxes) are unable to enhance expression from the cone promoter fragment. Blue and purple rectangles represent zebrafish UV opsin and rhodopsin promoter fragments respectively, upstream of EGFP. B-E. Confocal micrographs of retinal sections from 5 dpf embryos injected with 1.2 kb ZOP-EGFP or (3x PRE-1)-1.2 kb ZOP-EGFP constructs and stained with 4C12 and zpr-1 antibodies. EGFP-positive cells co localize (arrows) with rod photoreceptors (4C12 antibody), but not with cone photoreceptors (zpr-1 antibody). Nuclei are counter-stained with DAPI.