Figure 6

mRNA and protein levels of Egr-1 and luciferase after partial hepatectomy. Egr-1-luc mice were subjected to one third hepatectomy or sham operation, sacrificed 12 h (A) or 48 h (B) after surgery and liver tissue subjected to mRNA analyses by qRT-PCR (A) or Western blot analyzing protein levels of Egr-1 and luciferase (B).A: mRNA levels of Egr-1 and luciferase, respectively (average relative mRNA levels relative to 18S rRNA levels); mRNA levels of luciferase in sham operated animals were below the detection limit. n = 4, mean values + standard deviation are shown; *p < 0.05 sham vs. hepatectomy (U-test, Mann-Whitney). B: Representative Western blots showing the protein levels of Egr-1, luciferase and β-actin for sham operated (left panel) or hepatectomized animals (right panel), respectively; data from two representative animals per treatment are shown. Numbers indicate relative luciferase and Egr-1 expression calculated from optical densities (OD) of luciferase, Egr-1 and ß-actin protein bands. n = 4, mean values + standard deviation are shown; *p < 0.05 sham vs. hepatectomy (U-test, Mann-Whitney).