Figure 5
Immunohistochemical analyses of Egr-1 driven luciferase expression in regenerating liver. Egr-1-luc mice 4-8 weeks of age were subjected to one third liver hepatectomy as described in "Methods". Forty-eight hours after surgery mice were sacrificed, liver tissue fixed in PFA and stained for luciferase (luc). Tissue next to the site of surgery (rim upper left corner in both images) is shown and cells staining positive for luciferase (A) as well as Egr-1 (B) appear as clusters with brown-reddish staining (arrows; scale bar: 50 μm)