Figure 3

Immunohistochemical analyses of luciferase and Egr-1 expression during embryonic development. Egr-1-luc (A, B, C and G) and wildtype (D, E and F) C57BL/6 embryos on day E14 of development were stained for luciferase protein (A-F) or Egr-1 protein (G). In bone primordia of hindlimbs (A), sympathetic paravertebral ganglia (B) and masticatory apparatus (C) luciferase positive areas (arrows) are stained in lilac in transgenic embryos, in wildtype embryos no luciferase signal was detected (D-F). When staining for Egr-1 protein, a similar pattern of protein expression was found - exemplarily shown for the masticatory apparatus (G) - as for luciferase (C); scale bar: 50 μm