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Figure 4 | BMC Developmental Biology

Figure 4

From: The Enhancer of split transcription factor Her8a is a novel dimerisation partner for Her3 that controls anterior hindbrain neurogenesis in zebrafish

Figure 4

The heterodimerisation potential of full-length Her8a and Her3 proteins may account for their identical loss-of-function phenotypes. A. Co-affinity purification of full-length Her3 and Her8a proteins in HEK293T cells. When lysates from cells expressing both N-Strep/Flag-Her3 (N-SF-Her3) and Myc-tagged Her8a (myc-Her8a) proteins are eluted from a STREP-Tactin resin, myc-Her8a is observed to co-purify with N-SF-Her3 (lane 4). B-E. Schematized summary of the expression patterns (B) and loss-of-function phenotypes (C-E) of her8a and her3. Genes expression are color-coded and her5/her11 expression is indicated as a landmark (see also Figure 5H). Abbreviations: as in Figure 3; tg: trigeminal ganglion.

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