Figure 4

AP neurectodermal patterning is retained at 11 hpf in embryos lacking BMP signaling and an active organizer. Single in situ hybridization with rx3 (A,D,G,J,M) or fkd3 (B,E,H,K,N) probes or double in situ hybridization with both probes (C,F,I,L,O) is shown for wild-type embryos (A-C), untreated ich embryos (D-F), or ich embryos treated with bmp2bMO (G-I), the two β-cat MOs (J-L), or bmp2bMO plus the two β-cat MOs (M-O). In wild-type embryos, rx3 demarcates the eye-field (A,C) and fkd3 the diencephalon (arrows) and rhombomere 5 (arrowheads) of the hindbrain (B,C).