Figure 3

Regulation of bovine NOBOX expression by miR-196a in vitro in HeLa cells. (A) Representative Western blot showing specific suppression of bovine NOBOX by miR-196a in HeLa cells. β-Actin was used as loading control. The experiment was repeated four times with similar results, and a representative experiment is shown. (B) Semi-quantitative analysis of miRNA-196a regulation of NOBOX expression in transfected HeLa cells. Abundance of NOBOX protein in each sample was determined by densitometry and normalized relative to abundance of β-Actin protein (control). Data are expressed as mean relative pixel density (n = 4 mean ± SEM). Different letters indicate statistical difference (P < 0.05).