Figure 2

Spatial and temporal expression profile of miR-196a. (A) Tissue distribution of miR-196a analyzed by quantitative real-time PCR. Quantity of miRNA-196a was normalized to abundance of RPS18 mRNA and abundance expressed as relative fold change using the sample with the lowest value as the calibrator (n = 4 per tissue; mean ± SEM depicted). (B) Relative abundance of miR-196a in bovine oocytes and in vitro produced bovine early embryos (n = 4 pools of five oocytes/embryos each). Quantity of miRNA was normalized relative to abundance of miR-125b. The relative amount of miR-196a was expressed as relative fold change using the sample with the lowest value as the calibrator (n = 4, mean ± SEM). Different letters indicate statistical difference (P < 0.05).