Figure 8

Microinjecting HA-Pex11β RNA increased the number of peroxisome-like GFP-SKL structures during X. laevis embryogenesis. Both control (A and B) and HA-Pex11β injected (C and D) embryos at developmental stages 10 (A and C) and 20 (B and D), were fixed then sectioned for immunohistochemical analysis in somites for GFP-SKL. At stage 10, GFP fluorescence is present as a faint diffuse stain under control conditions (A), whereas following microinjection of HA-Pex11β punctate GFP structures could be seen at this early stage (C). At stage 20, punctate GFP structures were detected in both control and following microinjecting HA-Pex11β, where numbers were increased in the injected samples. An HA antibody was also used to confirm the ectopic presence of HA-Pex11β. DAPI (blue), GFP (green), HA-Pex11β (red), colocalization of HA-Pex11β and GFP-SKL (yellow). Images were taken at 60× magnification.