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Figure 7 | BMC Developmental Biology

Figure 7

From: PEX11β induces peroxisomal gene expression and alters peroxisome number during early Xenopus laevis development

Figure 7

Microinjecting HA-Pex11β RNA increased catalase immunofluorescence levels during X. laevis embryogenesis. Both control (A and B) and HA-Pex11β injected (C and D) embryos at developmental stages 10 (A and C) and 20 (B and D), were fixed then sectioned for immunohistochemical analysis in somites for catalase. At stage 10 catalase protein is undetected in somitic mesoderm under control conditions (A), whereas following microinjection of HA-Pex11β catalase protein is detectable in punctate structures (C). At stage 20, PMP70 protein was detected in both control and following microinjecting HA-Pex11β (B and D). An HA antibody was also used to confirm the ectopic presence of HA-Pex11β. DAPI (blue), PMP70 (green), HA-Pex11β (red), colocalization of HA-Pex11β and PMP70 (yellow). Images were taken at 60× magnification.

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