Skip to main content
Figure 6 | BMC Developmental Biology

Figure 6

From: PEX11β induces peroxisomal gene expression and alters peroxisome number during early Xenopus laevis development

Figure 6

Microinjecting HA-Pex11β RNA increased PMP70 immunofluorescence levels during X. laevis embryogenesis. Both control (A and B) and HA-Pex11β injected (C and D) embryos at developmental stages 10 (A and C) and 20 (B and D), were fixed then sectioned for immunohistochemical analysis in somites for PMP70. At stage 10 PMP70 protein is undetected in somitic mesoderm under control conditions (A), whereas following microinjection of HA-Pex11β PMP70 protein is detectable in punctate structures (C). At stage 20, PMP70 protein was detected in both control and following microinjecting HA-Pex11β (B and D). An HA antibody was also used to confirm the ectopic presence of HA-Pex11β. DAPI (blue), PMP70 (green), HA-Pex11β (red), colocalization of HA-Pex11β and catalase (yellow). Images were taken at 60× magnification.

Back to article page

BMC Developmental Biology

ISSN: 1471-213X

Contact us