Figure 5

Overexpression of HA-Pex11β did altered PPARα and γ, but not δ, gene expression during early X. laevis embryogenesis. The respective mRNA levels represent measures of mid-log phase RT-PCR product band intensities, relative to levels of EF1α. Using a similar approach and analysis as used in Figure 4, RT-PCR analysis of RNA isolated from control stage 10 embryos and HA-Pex11β injected embryos revealed significant changes in the expression of PPARα, and PPARγ, but not PPARδ. PPARα levels were elevated by treatment, while PPARγ levels were reduced. P < 0.05, n = 3. Values presented are the means ± SE.