Skip to main content
Figure 4 | BMC Developmental Biology

Figure 4

From: PEX11β induces peroxisomal gene expression and alters peroxisome number during early Xenopus laevis development

Figure 4

Embryonic overexpression of HA-Pex11β elevated Pex3, catalase and PMP70 levels. The respective mRNA levels represent measures of mid-log phase RT-PCR product band intensities, relative to levels of EF1α. RT-PCR analysis during normal embryogenesis revealed that the levels of all genes examined, with the exception of Pex5, increased as development progressed. First, repeated measures ANOVAs were carried out entering all RNA levels at all 3 stages. When significant, paired samples t-tests were carried out between stage levels in control embryos. This would reveal significant changes in RNA levels of the genes examined during normal development. Significant changes in RNA levels of a gene between stages is represented by the double asterisk ** (P < 0.05). Expression increases with development and there are differences in the levels of Pex3 and PMP70 between all stages, 10vs20, 20vs30 and 10 versus 30. For catalase there are differences between stages 10vs20 and 10vs30 but NOT between 20 versus 30. There are no significant differences in RNA levels between the tested developmental stages for Pex11β or Pex5. As the means were correlated, MANOVAs were carried out on the mean RNA levels of the treatment and control groups at each stage for each gene. All MANOVAs displayed significant effects of condition (Wilks lambda, P < 0.05) and so univariate ANOVAs were carried out. Treatments that resulted in significantly higher levels of gene expression following treatment are represented by a single asterisk. Pex11β, catalase and PMP70 all displayed significant increases in RNA levels all stage 10, stage 20 and stage 30 following treatment. Pex3 displayed elevated expression at only stage 10 and 30, while Pex5 displayed differences only at stages 20 and 30. (Ps < 0.05). n = 3. Values presented are the means ± SE.

Back to article page