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Figure 2 | BMC Developmental Biology

Figure 2

From: PEX11β induces peroxisomal gene expression and alters peroxisome number during early Xenopus laevis development

Figure 2

Overexpression of HA-Pex11β in A6 cells increased catalase and PMP70 protein levels. Western blotting revealed elevated proteins levels of catalase and PMP70 following the transfections of HA-Pex11β, but not GFP, in three samples of A6 cells (A). An HA antibody confirmed the translation and presence of HA-Pex11β in transfected cells (right 3 lanes) versus GFP transfected control cells (left 3 lanes). Catalase and PMP70 antibodies also displayed altered band intensities of each respective protein in HA-Pex11β transfected cells (right 3 lanes) versus GFP transfected cells (left 3 lanes). Protein loading in each lane was confirmed via a β-actin antibody. The Western blot signals were digitized and data were quantified and analyzed to statistically compare protein levels (B). There was a significant increase in the levels of catalase and PMP70 following overexpression of Pex11β, while there was no difference in the levels of β-actin. Statistical relevance of discrepancies between groups (asterisks) was assessed by an independent samples t-test. P < 0.05, n = 3. Values presented are the means ± SE.

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