Figure 1

Overexpressing HA-Pex11β altered peroxisome related gene expression in Xenopus A6 cells. RT-PCR analysis of peroxisomal genes was performed before and after transfection of A6 cells with HA-Pex11β. Two days following transfection 250 ng of reverse-transcribed A6 cell RNAs from control and treatment samples (n = 3) were subject to PCR amplification using specific primers for the peroxisome related genes; Pex11β, PMP70, catalase, Pex5, Pex3, Pex1, PPARα, -δ, and -γ. The respective mRNA levels represent measures of mid-log phase RT-PCR product band intensities, relative to levels of EF1α. Genes whose levels were altered significantly, as assessed by an independent samples t-test, are denoted with an asterisk. Pex11β, PMP70, catalase, Pex5 and PPARα displayed elevated levels of expression following treatment, while PPARγ displayed reduced expression. P < 0.05, n = 3. Values presented are the means ± SE.