Figure 2

Sections of early Hislopia malayensis buds. (a) Cross-section through an early bud showing the prominent inner and outer budding layer. Asterisk marks a coelomocyte within the body cavity. (b) Slight oblique section through budding stage 3 showing dense peritoneal cells accumulating at the developing lophophoral base. (c) Cross-section through the vestibular wall of budding stage 5 showing the first signs of collar formation in the vestibulum. Abbreviations: am - apertural muscles, c - developing collar, cw - cystid wall, epl - epidermal layer of the cystid, ga - gut anlage, ibl - inner budding layer, la - lophophore anlage, lb - lumen of the bud, lca - lophophoral coelom anlage, nb - neck of the bud, obl - outer budding layer, pl - peritoneal layer of the cystid, pma - prospective mouth area, ts - tentacle sheath, vw - vestibular wall. Scale bar in (a) and (c) = 30 μm, in (b) 50 μm.