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Figure 4 | BMC Developmental Biology

Figure 4

From: Lipid rafts enriched in monosialylGb5Cer carrying the stage-specific embryonic antigen-4 epitope are involved in development of mouse preimplantation embryos at cleavage stage

Figure 4

Pretreatment with MbCD inhibits cytokinesis and development of mouse preimplantation embryos. (A) Effect of MbCD on the expression of MSGb5Cer (green), GM1 (red) and E-cadherin (red) on 8-cell embryos. The left column and the right column of each panel represent a 2D image and the overlay of a 2D image and a differential interference contrast (DIC) micrograph, respectively. Thirty 8-cell embryos were used for the experiment represented in the images. (B) Representative DIC images of embryos cultured for 0, 24 and 48 hours in M16 medium after preincubation with 0-5 mM MbCD. Scale bars: 100 μm. Twenty 8-cell embryos were used for the experiment represented each images. (C) The percentages of living embryos cultured for 12, 24 and 48 hours after preincubation with 0-5 mM MbCD. Twenty 8-cell embryos were cultured for each concentration of MbCD.

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BMC Developmental Biology

ISSN: 1471-213X

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