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Figure 1 | BMC Developmental Biology

Figure 1

From: Lipid rafts enriched in monosialylGb5Cer carrying the stage-specific embryonic antigen-4 epitope are involved in development of mouse preimplantation embryos at cleavage stage

Figure 1

MSGb5Cer localize at the interfaces between blastomeres in mouse preimplantation embryos. (A) Localization of MSGb5Cer, E-cadherin and GM1 in an unfertilized egg and an embryo at the zygote, 2-cell stage and 8-cell stage. MSGb5Cer (top row), E-cadherin (middle row), and GM1 (bottom row) in a living mouse egg or embryos were stained with Alexa Fluor® 488-conjugated 6E2, ECCD-2/Alexa Fluor® 546 Rabbit Anti-rat IgG, and biotinylated-CTX B/Alexa Fluor® 546 streptavidin, respectively, and examined. Thirty sample unfertilized egg and 45 sample embryos of other stages were used for this experiment represented in image. (B) Distribution of BODIPY®FL-MSGb5Cer and BODIPY®FL-GM1 in a 2-cell stage embryo. The distribution of MSGb5Cer and GM1 is shown in the form of optical slice images. BODIPY®FL-C12 fatty acid was used as a negative control. All scale bars represent 20 μm. Ten sample 2-cell stage embryos were used for this experiment represented in each image. (C) Distribution of BODIPY®FL-MSGb5Cer in a 2-cell stage embryo cultured in the presence of 6E2. The BODIPY®FL-MSGb5Cer introduced 2-cell embryo was cultured for 1 hour in M16 medium containing 15 μg/ml of 6E2, and then stained with Alexa Fluor® 546-conjugated 6E2. Scale bar represent 20 μm. Ten sample 2-cell stage embryos were used for this experiment represented in image.

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BMC Developmental Biology

ISSN: 1471-213X

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