Figure 4

E- and N-cadherin expression in control and SU5402 treated embryos. A-B: Brightfield images of control (A) and SU5402 treated (B) embryos that were processed for immunofluorescence analysis of E-cadherin (ECad) and N-cadherin (NCad). A'-A'": transverse section showing the same microscopic field at the indicate streak level of the control embryo in A, visualizing E-cadherin protein (A'), N-cadherin protein (A"), and E-cadherin plus N-cadherin (A'"). B'-B'": transverse section showing the same microscopic field at the indicate streak level of the SU5402 treated embryo in B, visualizing E-cadherin protein (B'), N-cadherin protein (B"), and E-cadherin plus N-cadherin (B'"). C-D: higher magnification views of the boxed areas in A' and B'. E-H: Whole mount ISH visualization of N-cadherin (E, F) and E-cadherin (G, H) mRNAs in control and SU5402 treated embryos. I: Realtime RT-PCR analysis showing relative E-cadherin and EPHA1 mRNA levels in control versus SU5402 treated primitive streak region (preingression epiblast, primitive streak, medial mesoderm) and isolated preingression epiblast. Error bars indicate standard deviation. Abbreviations: Mes, mesoderm; End, endoderm.