Figure 1

Microarray comparisons of genomic DNA from Ax2, Ax4 and cdk8^- _HL strains. The log(2)ratio of abundance of each gene in each comparison is plotted against the position of that gene on the chromosome. Ax2P is the parental strain used to generate cdk8^-_HL and Ax2K is the Ax2 strain with minimal genomic duplications [14]. Shown are comparison of genes on Chromosome 2 between Ax2K and cdk8^-_HL, and between Ax2P (the parent of cdk8^-_HL) and Ax4, and a comparison of genes on chromosome 5 for Ax2K and cdk8^-_HL. The approximate position of the putative cdk8^-_HL duplications relative to Ax2K are marked with grey horizontals bars. Logratios of approximately 1 or -1 indicate 2-fold changes in copy number (duplications); logratios around zero indicate equal copy number. The known Ax4 duplication is marked with a red horizontal bar; logratios in this region average approximately zero in this comparison show that both strains have the same copy number here. Ax2P has a longer duplication than that apparent in Ax4, though in the same region, as represented by the grey bar in the middle panel. The cdk8 gene is found on chromosome 1 and so its loss is not apparent on the comparisons shown here.