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Figure 7 | BMC Developmental Biology

Figure 7

From: Serum response factor is required for cell contact maintenance but dispensable for proliferation in visceral yolk sac endothelium

Figure 7

SRF-null VYS mesoderm tissues show aberrant proliferation. Immunodetection of BrdU incorporation was used to determine the proliferation status of VYS tissues. Double-label immunofluorescence images of VYS tissue from wild type (A, B) and SRF-null (C, D) embryos at E12.5. Tissues were stained for SRF (green), BrdU (red), and DNA (blue); monochrome images in B and D show BrdU staining in isolation. Arrows in A and B highlight SRF-positive nuclei that colocalize with BrdU; arrowheads in C and D mark nuclei lacking SRF that stain positively for BrdU. Dashed line indicates division between Ed and Md layers. Magnification = 200×, scale bar = 50 μm; Ed = endoderm, Md = mesoderm. (C) Cytometric analysis of BrdU-positive nuclei in E10.5, E11.5 and E12.5 VYS mesoderm. Values are expressed as a percentage of total DAPI-stained nuclei counted. *p = 0.025; **p = 0.007.

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