Figure 1
SRF protein is lost in VYS mesoderm endothelial cells expressing Tie2-Cre recombinase. Double-label immunofluorescence images of E12.5 VYS tissue from wild type (Tie2Cre-/-·Srff/f·ROSA26R-eYFP+/+; A, C) and SRF-null (Tie2Cre+/0·Srff/f·ROSA26R-eYFP+/+; B, D) embryos. (A, B) Immunostaining of eYFP by anti-GFP antibody was used to detect evidence of functional Tie2-Cre expression. Tissues in A & B were stained for SRF (green), GFP (red), and DNA (blue). Arrows in A highlight SRF-positive nuclei in VYS mesoderm; arrowheads in B highlight GFP positive cells in VYS mesoderm. (C, D) VE-Cad labels endothelial cells in VYS mesoderm. Tissues in C & D were stained for VE-Cad (red) and DNA (blue). Arrow (C) and arrowheads (D) indicate positive VE-Cad staining. Magnification = 200×, scale bars = 50 μm; Ed = endoderm, Md = mesoderm.