Figure 8

ERK and dMP1 bind polytene chromosomes where they partially co-localize with Corto. (A): Immunostaining of polytene chromosomes using rabbit anti-ERK (C-16) (left), and guinea-pig anti-dMP1 (center). DNA was stained with DAPI. ERK and dMP1 co-localize on all sites (right). The staining observed with dMP1 antibodies on centromeric heterochromatin and chromosome four (asterisk) is non-specific, since the same staining was observed with the pre-immune serum (data not shown). (B): Immunostaining of polytene chromosomes with rabbit anti-Corto antibodies (left) and guinea-pig anti-dMP1 antibodies (center). Corto and dMP1 co-localize on a number of sites (right, arrows). (C): Magnification of chromosome 3L extremity showing that Corto and dMP1 co-localize on several sites (yellow arrows).