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Figure 5 | BMC Developmental Biology

Figure 5

From: The MAP kinase ERK and its scaffold protein MP1 interact with the chromatin regulator Corto during Drosophilawing tissue development

Figure 5

Corto is a phosphorylated protein located both in the cytoplasm and in the nucleus. (A to C): Corto is present in the cytoplasm and in the nucleus and exhibits several isoforms very close in size (indicated by a brace). In the nucleus, the higher isoforms are predominant. (A, B): Western-blot using anti-Corto antibodies and cytoplasmic or nuclear extracts from wild-type embryos (A) or S2 cells (B). (C): Western-blot with anti-FLAG antibodies using cytoplasmic and nuclear extracts from S2 cells expressing or not a Corto-FLAG fusion protein. Cyt: 50 μg cytoplasmic extract; Nuc: 10 μg nuclear extract. In A, B, C, β-tubulin and lamin were used as cytoplasmic and nuclear loading controls, respectively. (D): The different Corto isoforms correspond to proteins that are differently phosphorylated: Western-blot with anti-FLAG antibodies after phosphatase treatment of 15 μg total extract from S2 cells expressing a Corto-FLAG fusion protein shows that the upper band disappears. In this experiment, only the two major Corto-FLAG isoforms are visible because of the low amount of total extract loaded.

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