Figure 3

Functional characterization of PARP antisense (As). (A) RT-PCR of PARP down-regulated D. discoideum cells. Gene specific expression of parp exhibits ~60% reduction while no change was observed with internal control rnlA. (B) Densitometric analysis of PARP expression by RT-PCR. (C) PARP activation monitored in PARP down-regulated D. discoideum cells by indirect immunofluorescence. PARP activity in down-regulated cells was found to be reduced compared to the control. (D) Densitometric analysis of PARP activity. ** p value <0.01; *p value <0.05 compared to control. The fluorescence photographs have been taken under 60X objective.