Figure 4

The expression pattern of 18 candidate genes, whose expression is increased, at d8DE, in E8.5 mouse definitive endoderm, analyzed by in situ hybridization. (A) The expression of 16 candidate genes, whose expression is increased >5 fold in d8DE Pdx1/GFP+ population, (B) >5 fold in d7DE versus d5DE, or (C) >2 fold in d8DE Pdx1+ versus Pdx1- cells, are shown. Gene names are indicated at top of the pictures. Arrows indicate the sites detected. Cross sections through the white line of Palld1 or Zmiz1 are shown (right panels). A: anterior; P: posterior. Scale bars: 100 μm