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Figure 1 | BMC Developmental Biology

Figure 1

From: An expression profile analysis of ES cell-derived definitive endodermal cells and Pdx1-expressing cells

Figure 1

Microarray analysis of ES cell-derived cells. (A) ES cells and ES cell-derived cells were isolated by the expression of cell surface antigen as previously described [14]. Populations isolated were: ES cells (ES), ectoderm (ECT), mesendoderm (MES), lateral plate mesoderm, (LPM); Paraxial mesoderm, (PAM) and DE at day 5 (D5), day 7 (D7) and day 8 (D8, DE Pdx1-, DE Pdx1+). (B) Clustering of gene expression in ES cells, ECT, LPM, PAM, MES, d5DE, d7DE, d8DE Pdx1- and d8DE Pdx1+ cell lineages. Each line indicates an individual gene. Red lines indicate genes with high expressions and green lines indicate genes with low expressions in the DE lineages. Y axis represents normalized value of the expression level. (C) Microarray results and real time PCR analyses of six representative candidate genes, namely, AI464131, Akr1c19, Foxp4, Hipk2, Pcbd1, and Tmem184a, together with Sox17, and Pdx1, as positive controls. Right Y-axis values represent GeneChip signal intensities of candidate genes. Transcription levels of the representative candidate genes in each population are quantified by real-time PCR analysis. The transcription levels are normalized with that of b-actin. The values are further normalized with that of Pdx1-positive DE on d8 (d8DE GFP+) and thus the left Y-axis values represent relative gene expression levels when the expression level at d8 (d8DE GFP+) is defined as 100.

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