Figure 4
From: Notch signaling regulates remodeling and vessel diameter in the extraembryonic yolk sac
Gene expression in transgenic EC-N1ICD and EC-Rbpj-KO embryo yolk sacs. Semiquantitative RT-PCR analysis was used to confirm gene expression differences seen in whole genome microarrays. (A) RT-PCR analysis of Hey1 and Heyl in wild type, EC-N1ICD, and EC-Rbpj-KO E9.5 yolk sacs. Quantitated relative mRNA levels are shown in the lower graph. (B) RT-PCR analysis of genes critical for the formation of the early embryonic vasculature. Quantitated relative mRNA levels of select genes are shown in the graphs to the right. * p < .05, ** p < .01, *** p < .001 compared to wild type.