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Figure 2 | BMC Developmental Biology

Figure 2

From: Notch signaling regulates remodeling and vessel diameter in the extraembryonic yolk sac

Figure 2

Defects in growth and yolk sac vasculature remodeling in EC-N1ICD and EC-Rbpj-KO embryos. (A-C) Lateral view of E9.5 wild type (A), EC-N1ICD (B), and EC-Rbpj-KO (C) embryos. EC-N1ICD and EC-Rbpj-KO embryos were smaller in size than the wild type and exhibited cardiovascular defects. (D-F) Whole mount E9.5 wild type (D), EC-N1ICD (E), and EC-Rbpj-KO (F) embryos with surrounding yolk sac. The EC-N1ICD and EC-Rbpj-KO yolk sac lacked the large, well-defined blood vessels seen in the wild type. The blood in the EC-N1ICD yolk sac collected near the attachment to the placenta. (G-I) Immunofluorescence image of E9.5 wild type (G), EC-N1ICD (H) and EC-Rpbj-KO (H) yolk sac visualized with an antibody to PECAM1. Wild type embryos showed a remodeled yolk sac vasculature, with both large and small caliber vessels. The EC-N1ICD yolk sac did not exhibit branching; all vessels were of a large caliber. The EC-Rbpj-KO yolk sac also failed to show vascular remodeling, although all vessels were of a small caliber. Scale bars are 500 μm (A-C), 1 mm (D-F) and 100 μm (G-I). Arrows (G-I), avascular inter-vessel space. Asterisk (G), capillary collapse.

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