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Figure 5 | BMC Developmental Biology

Figure 5

From: Conditional expression of retrovirally delivered anti-MYCN shRNA as an in vitro model system to study neuronal differentiation in MYCN-amplified neuroblastoma

Figure 5

Induced anti-MYCN shRNA expression decreases MYCN and increases GAP43 expression in MNA neuroblastoma cells. (A) A representative western blot analysis of MYCN, GAP43 and β-actin protein expression in Kelly and SK-N-BE(2) cells induced to express the aMN-1658 shRNA. Real-time RT-PCR analysis of MYCN mRNA (B) and aMN-1658 shRNA (C) expression was performed on total RNA isolated from Kelly and SK-N-BE(2) cells treated as described. Cells were transduced with retrovirus RV-1658 and incubated for the indicated numbers of days in the presence (+) or absence (-) of 1 μg/ml doxycyclin (dox). 3/3 and +/- indicate that the cells were incubated for 3 days in the presence of dox, followed by 3 days in the absence of dox. Error bars indicate SDs.

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