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Figure 3 | BMC Developmental Biology

Figure 3

From: Conditional expression of retrovirally delivered anti-MYCN shRNA as an in vitro model system to study neuronal differentiation in MYCN-amplified neuroblastoma

Figure 3

Transient MYCN knockdown in MNA Kelly and SK-N-BE(2) cells using shRNA technology. (A): A representative western blot analysis of MYCN protein expression in Kelly and SK-N-BE(2) cells transiently transfected for 3 days with plasmids expressing the aMN-887 and aMN1658 shRNAs. β-actin expression was used for normalization. Quantitative real-time RT-PCR was used to investigate the expression of MYCN (B), c-MYC (C), neurofilament light-chain - NFL (D) and growth-associated protein 43 - GAP43 (E) mRNAs. Error bars indicate SDs. (F) and (G): Flow cytometric analyses showing the cell cycle distribution of SK-N-BE(2) and Kelly cells transiently transfected with the aMN-887 and aMN-1658 shRNA expressing plasmids. Scr indicates the scrambled shRNA controls.

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BMC Developmental Biology

ISSN: 1471-213X

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