Figure 1From: Conditional expression of retrovirally delivered anti-MYCN shRNA as an in vitro model system to study neuronal differentiation in MYCN-amplified neuroblastomaRetrovirally delivered inducible shRNA expression in HEK293 cells. TetR-expressing HEK293 cells (HEK293T-Rex) were transduced with retroviruses expressing anti-Luciferase (a-Luc) or scrambled (Scr) shRNAs. The a-Luc shRNA was expressed from a wt-H1 or the inducible H1-2O2 promoter in the absence (-) or presence (+) of 1 μg/ml doxycyclin (dox) for 48 hrs. Error bars indicate SDs.Back to article page