Figure 1

Expression of cardiac transcripts and proteins in cardiomyocytes derived from human iPS and ES cells. A. qRT-PCR assessment of expression of cardiac specific genes NKX2.5, MLC2v, ACTN2, MYH6, GATA4 and a skeletal muscle gene MYH2 in undifferentiated iPS and ES cells and their corresponding beating clusters (BC) microdissected at day 18 of differentiation. The data are means of triplicate analyses ± SEM. B. Anti-sarcomeric cardiac actinin and cardiac troponin T stainings in HES2- (upper panels) and iPS(Forskin) C1-derived (lower panels) cardiomyocytes. Spontaneously beating outgrowths were microdissected at day 15 of differentiation and replated on fibronectin-coated plates. Adherent beating clusters were used for staining 3-5 days later. Scale bar: 20 μm.