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Figure 5 | BMC Developmental Biology

Figure 5

From: Phosphatidylinositol 3 kinase modulation of trophoblast cell differentiation

Figure 5

PI3K impact on trophoblast cell differentiation: morphology and DNA content. Morphology and DNA content were assessed in Rcho-1 trophoblast stem cells cultured in the following conditions: stem (Stem), differentiating (Dif), differentiating with vehicle exposure (0.1% DMSO; Dif+V), or LY294002 (10 μM; Dif+LY). A) Morphology was determined by bright field microscopy (top panels). Actin filaments were stained with rhodamine-conjugated phalloidin; nuclei were visualized with DAPI (bottom). Bar= 50 μm. B) DNA content was estimated by propidium iodine staining followed by flow cytometry. Due to the tetraploid nature of the Rcho-1 trophoblast cells, 4N and 8N cell populations correspond to dividing cells, whereas cell populations with more than 8N DNA content have undergone endoreduplication. C) Graphic representation of three independent flow cytometry experiments. Student's t-tests (*P < 0.05).

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