Figure 4

The effects of mtmr8 knockdown on vascular marker genes and vascular endothelium. (A-J) Whole mount in situ hybridization with the vascular marker gene-specific probes (as shown on the left) in Cont-MO (A, C, E, G and I) and mtmr8-MO (B, D, F, H and J) embryos at 26 hpf. The signals of DA, PCV and Se are indicated by arrows, and the PCV expansion size is indicated by double-arrowheads in the flt4 probe hybridization (E and F). (K-N) Lateral (K, M) and dorsal (L, N) views of alkaline phosphatase-stained Cont-MO (K, L) and mtmr8-MO (M, N) embryos at 3 dpf.