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Figure 8 | BMC Developmental Biology

Figure 8

From: The essential role of bursicon during Drosophiladevelopment

Figure 8

Phenotypes resulting from different GAL4 drivers of UAS-rk RNAi. (A) All T76>rk RNAi progeny die with small, soft, untanned puparia before pupal ecdysis. The puparia often have a slight crescent bend to them, rather than a straight orientation along the anterior-posterior axis. (B) In contrast, T80>rk RNAi develop into pharate adults, but all are then unable to eclose from their puparia and die. (C) A limited number of 69B>rk RNAi flies are able to eclose, but their legs are flaccid and collapse under the weight of the body. All flies die within 24 hours, before ever expanding their wings or tanning their cuticle. (D) C855a>rk RNAi flies successfully eclose without any obvious problems. However, all adults have drooping, partially extended wings. This appears to be a result of the successful deployment of wing expansion behaviors in the absence of cuticular tanning. Only A and B are shown at the same scale, to emphasize the smaller size of T76>rk RNAi pupae.

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