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Figure 4 | BMC Developmental Biology

Figure 4

From: The essential role of bursicon during Drosophiladevelopment

Figure 4

Hormone release from type III boutons at the pupal NMJ coincides with pupal ecdysis. Changes in fluorescence intensity are also observed in type III boutons of CCAP>ANF-EMD animals at pupal ecdysis. (A) P3 stage pupa, preceding pupal ecdysis by several hours. (B) Pupa at end of P4(i) stage, shortly after initiation of the ecdysis sequence. (C) P4(ii) stage pupa immediately following head eversion (occurring during pupal ecdysis). (D) P5(i) pupa following elongation of legs, approximately 1/2 hour after head eversion. Average fluorescence intensity is reported on a log scale. For (D), no detectable fluorescence intensity is reported. Identification of pupal stages is described in the Methods. Error bars indicate +/- SEM. Scale bar = 10 μm.

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