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Figure 2 | BMC Developmental Biology

Figure 2

From: The essential role of bursicon during Drosophiladevelopment

Figure 2

Bursicon co-localizes with the pre-synaptic markers N-SYB and CSP at the NMJ. (A) Neuronal synaptobrevin (N-SYB) expression at NMJ 12 includes type III boutons. (Aa) BURS-IR in type III boutons. (Ab) Distribution of N-SYB-IR at NMJ 12. (Ac) N-SYB is expressed in multiple bouton types, including type III. (B) Cysteine string protein expression pattern at NMJ 12 includes type III boutons. (Ba) BURS-IR as a marker for type III boutons. (Bb) CSP-IR labels multiple boutons at NMJ 12. (C) BURS and CSP patterns co-localize in type III boutons. The expression of CSP immunoreactivity in type III boutons appears to be weaker than in other boutons at NMJ 12. In (A) and (B), arrows indicate representative boutons that co-express BURS and the relevant pre-synaptic marker, whereas arrowheads indicate boutons that do not express bursicon. Scale bars = 10 μm.

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